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Welcome to EMBL Flow Cytometry site! |
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Welcome to the Flow Cytometry Core Facility at EMBL Heidelberg. The aim of this website is to serve as a content rich resource for the staff at EMBL and beyond. We have a number of downloadable resources including software, hardware designs, teaching tutorials and how-tos and educational resources such as video tutorials. We have developed experimental setup recording documents that will keep track of users experiments on a MoFlo. We aim to keep this website regularly updated with new information and resources, so please come back and visit. The Flow Cytometry Core Facility offers a range of flow cytometric techniques. The equipment adds flexibility in the preparation and execution of experiments allowing different approaches to research problems. Our facility meets researchers' needs and enables better resolution in terms of analysis and product. The goal of the facility is to proactively introduce flow cytometric methods into new research areas while supporting and extending current research. Please check our Flow Cytometry Core Facility link at EMBL website. You might also like to visit other EMBL Core Facilities websites. |
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Rmax in evaluations of sorting performance |
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Cell sorter’s performance is assessed by well establish parameters such as Purity, Yield and Recovery. Purity is a check on the quality of the sort decisions made by the instrument. Yield establishes how well the instrument is at getting the target particles from the original sample. Recovery has been used to defined either Yield or the accuracy of sort counters. Measuring these parameters require counting particles in the original or the sorted sample, which might not be feasible when dealing with rare populations or precious samples We propose an alternative parameter Rmax to estimate Recovery that exclusively relies on the frequency of sort target and non-target populations in the original sample and in the centre stream catch. This simple method can be performed with an ideal sample based only on beads in systematic comparisons of performance under different sorting conditions (sort decision mode, drop drive frequency, nozzle size, etc).It could also be used to identify and troubleshoot sort problems based on deviations relative to the ideal recovery during cell sorts. Check our poster on Rmax , winner of the best poster prize during the FlowcytometryUK 2011 meeting. |
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European Cytometry Network is up and running! |
 The inaugural meeting of the European Cytometry Network (ECN) took place in EMBL-Heidelberg on the 28-29 February 2008. Following discussions held at that meeting, the basic ECN key aims were outlined. We introduced the idea of a Web2.0 networking platform as the infrastructure to enhance communication, education, scientific exchange and collaboration among European Cytometrists. Encouraged by the overal support for this idea, the first drafts of the platform were soon implemented. A year has past and the ECN is up and running as a networking platform to connect cytometrists all over Europe and beyond.  Figure 1. The European Cytometry Network membership by countries. Absolute numbers and country distribution of participants at the inaugural meeting is shown in A. Relative frequency of registered members at eurocyt.net (30-01-2009) shown in B. Also in B, '*' signifies the National Societies represented in the ECN. |
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European Cytometry Network Meeting report |
 A report on the the First European Cytometry Network Meeting (28-29th February 2008) is now available for your perusal. The report covers findings from a survey on European cytometrists that provides useful information on the current state of our community. This information is particularly useful to us in defining the right platform for the backbone of the network. However, we are still open to feed back at this critical time and, again we ask, for your input. You can also contribute with ideas to this Network by filling the survey pdf accesible here. When complete, please send it to the following address: Flow Cytometry Core Facility EMBL Meyerhofstr. 1, Heidelberg D-69117 Germany |
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